Reverse lectin ELISA for detecting fucosylated forms of α1-acid glycoprotein associated with hepatocellular carcinoma
A. Binding of different concentrations of purified AGP from patient samples to S2 in the reverse S2 lectin ELISA. AGP purified from a patient with HCC (black triangles), AGP purified from a cirrhosis patient (black squares) and AGP purified from a normal sample (black circles). B. Comparison of binding of different concentrations of purified AGP from a HCC patient sample diluted in 1% BSA in PBS (circles) and diluted in normal serum (black squares). C. Binding of different concentrations of dsAGP to S2 in the reverse S2 lectin ELISA assay.