AMPK modulatory activity of olive–tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach
(A) Quantitative assessment of lipid vesicles in hypertrophic adipocytes incubated with 400, 600, or 800 μg/mL of Olive-tree leaves extract and compared to the control in a high glucose medium. Values have been normalized with respect to the control incubated in a high glucose medium. ** p<0.01 indicates significant differences compared to the control. Representative microphotographs for the qualitative assessment of 3T3-L1 lipid droplets: hypertrophic adipocytes differentiated for 22 days, phase contrast (panel B), same cells stained for triglycerides in lipid droplets (green, panel D) and nuclei stained with DAPI to show the localization of nuclear DNA (blue, panel C). Superimposed lipid droplets and cellular nucleus (panel E).