Mated Drosophila melanogaster females consume more amino acids during the dark phase
The oviposition assay was performed for 12 h starting at either ZT 0 (for the light phase) or ZT 12 (for the dark phase). L and D represent results obtained during the light (orange bars) and dark (blue bars) phases, respectively; filled bars represent CS flies; hatched bars represent per0 flies. (A) The mean number of eggs laid during L and D phases on aa(-) medium by an amino acid-deprived female (n = 20). (B) The mean number of eggs laid during L and D phases on a standard cornmeal-agar-yeast-glucose medium (SM) by a female raised on SM (n = 20). Error bars indicate SEM. **p < 0.01 and ***p < 0.001 for comparisons between L and D phases using the Student’s t-test.