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Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water

Fig 4

Measurement of protein radicals of ERW and dissolved H2 water by immuno spin trapping assay.

A. 60 mM DMPO and sample water were applied for 90 min after treatment with 80 μM H2O2 for 30 min. B. 40 mM DMPO, sample water and 80 μM H2O2 were applied at the same time for 90 min. FW, filtered pure water; +Mixed gas, H2, O2 and N2 mixed gas were bubbled through water to obtain DO and DH concentrations equal to those of LV4 –the pH was also adjusted using sodium hydrate; LV4, ERW at electrolysis level 4. NAC, 5 mM N-acetyl cysteine. *a, *b indicate p values of < 0.01 when compared with the values for H2O2-treated FW and +Mixed gas FW, respectively.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0171192.g004