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Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water

Fig 3

Measurement of intracellular ROS intensity of degassed and autoclaved controls and ERW using BES-H2O2 probe.

A) Comparison of degassed sample water vs. ERW. B) Comparison of autoclaved sample water vs. ERW. MQ, MilliQ water; FW, filtered pure water; +Mixed gas, H2, O2 and N2 mixed gas was bubbled through water to obtain DO and DH concentrations that are equal to those of LV4. The pH was also adjusted using sodium hydrate. LV4, ERW at electrolysis level 4. NAC, 5 mM N-acetyl cysteine. Degas, Degassed samples. Autoclave, autoclaved samples. *a and *b indicate p values of < 0.01 when compared with the value for H2O2-treated MQ and degassed +Mix gas of FW, respectively.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0171192.g003