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Biofilm Formation by Clostridium ljungdahlii Is Induced by Sodium Chloride Stress: Experimental Evaluation and Transcriptome Analysis

Fig 2

Effect of the NaCl concentration on biofilm formation and growth.

A) C. ljungdahlii was grown in well plates and NaCl was added to the medium in concentrations ranging from 0 to 280 mM (n = 3). The crystal violet assay (described in text) was performed after 3 days of incubation. The pictures underneath the data bars show the corresponding, stained biofilms, before extraction with methanol. Remark that OD 600 nm values are given on a linear scale, while the A 570 nm values are on a logarithmic scale. B) C. ljungdahlii was grown in tubes and NaCl was added to the medium in concentrations ranging from 0 to 280 mM (n = 3). Tubes were vortexed at a low speed to break up the formed aggregates, before measurement of the OD 600 nm.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0170406.g002