Comparison of Enzymatic Traits between Native and Recombinant Glycine Sarcosine N-Methyltransferase from Methanohalophilus portucalensis FDF1T
Fig 2
GSMT activities were activated by monovalent cations but inhibited by the end product betaine and the competitive inhibitor SAH.
Assay mixtures contained 0.1 M TES, pH 7.3, 1 mM SAM (containing 0.1 μCi/2 pmol [3H-methyl]-SAM) and 0.5 M glycine or sarcosine under various concentrations of KCl (A) or NaCl (B). The inhibition assays were carried out under 1.0 M KCl with various concentrations of betaine (C) or SAH (D). All data points were averaged from three independent experiments.