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Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm

Fig 5

DNA comet images in the hemocytes of the 5th instar of S. exigua from the control and cadmium strains. After isolation the cells were suspended in PBS and mixed with a H2O2 solution (treated groups; final concentration 50 μM) or with PBS (reference groups) and incubated for 1 min.

Abbreviations: Variant 0 –individuals from the control and cadmium-treated strains through 120 generations; Variant 1 –individuals from the control and cadmium strains that were fed the exchanged diet (C->Cd: control to cadmium or Cd->C: cadmium to control) for one week. Variant 2 –individuals from the control and cadmium strains that were fed the exchanged diet for one generation; Time—time period (0, 5, 15 or 30 min) after the end of the incubation with H2O2 or PBS.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0167371.g005