Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
Mice were given i.p. injections of 2x108 rat erythrocytes weekly for 5–6 weeks to induce AIHA. Mice were sacrificed 3 days after 5th and 6th injections and their BM and spleen cells were harvested. Cells isolated were stained with anti-mouse CD71-PE, anti-mouse Ter119-APC and 7AAD, and incubated with CM-H2DCFDA. The erythroid cells at different stages of maturation were determined and ROS generation was estimated in each of them. ROS in erythroid cells at different stages of maturation are given above. Each value represents mean ± SEM of observations. n = 4 control and 6 AIHA-induced mice. *p<0.05, **p<0.01 and ****p<0.001 for comparison of the groups (Student t-test).