Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
Mice were given weekly i.p. injections of rat erythrocytes to induce AIHA. Mice were sacrificed 3 days after 6th injection and their BM and spleen cells were harvested. Cells isolated were stained with anti-mouse CD71-PE, anti-mouse Ter119-APC and 7AAD, and proportion of erythroid cells were determined as described in S3 Fig. Proportion of erythroid cells in different maturational stages of development in BM and spleen in control and AIHA mice are given along with percent changes in AIHA (ANOVA, p<0.001 for both BM and spleen). Each value represents mean ± SEM of observations. n = 4 control and 6 AIHA-induced mice. *p<0.05, ***p<0.005 and ****p<0.001 for comparison of the groups (Student t-test).