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Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse

Fig 6

Total erythroid cells in control and AIHA induced mice.

Mice were given i.p. injections of 2x108 rat erythrocytes weekly for 5–6 weeks to induce AIHA. Mice were sacrificed 3 days after 5th and 6th doses of injection and their BM and spleen cells were harvested. Cells isolated were stained with anti-mouse CD71-PE, anti-mouse Ter119-APC and 7AAD, after blocking with anti-mouse CD16/32, and the proportions of erythroid cells (as described in S3 Fig) were determined. Proportions of erythroid cells in BM and spleen of mice are given above (BM p<0.001 and spleen p = 0.061, ANOVA). Each value represents mean ± SEM of observations. n = 4 control and 6 AIHA mice. *p<0.05, ***p<0.005 and ****p<0.001 for comparison of the groups (Student t-test).

Fig 6