Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
Mice were given i.p. injections of 2x108 rat erythrocytes weekly for 5–6 weeks to induce AIHA. Mice were sacrificed 3 days after 5th and 6th doses of injection and their BM and spleen cells were harvested. Cells isolated were stained with anti-mouse CD71-PE, anti-mouse Ter119-APC and 7AAD, after blocking with anti-mouse CD16/32, and the proportions of erythroid cells (as described in S3 Fig) were determined. Proportions of erythroid cells in BM and spleen of mice are given above (BM p<0.001 and spleen p = 0.061, ANOVA). Each value represents mean ± SEM of observations. n = 4 control and 6 AIHA mice. *p<0.05, ***p<0.005 and ****p<0.001 for comparison of the groups (Student t-test).