Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
DIB labeled mice were given weekly i.p. injections of 2x108 rat erythrocytes for 5–6 weeks to induce AIHA. Blood samples were collected from control and AIHA mice 3 days after the 5th and 6th injection. Erythrocytes were stained ex vivo with anti-mouse IgG-PE, streptavidin-APC and anti-mouse CD71-FITC. Erythrocytes of different age groups were gated and autoantibody level was analyzed in each of them. Each value represents mean ± SEM of data. n = 8 control and 12 AIHA mice. *p<0.05, **p<0.01, ***p<0.005 and ****p<0.001 for comparison of the groups (Student t-test). ANOVA test for autoantibody binding on different age groups of erythrocytes significant (p = 0.001 in terms of MFI and p = 0.021 in terms of erythrocyte proportion with membrane-bound autoantibody).