Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
Mice were injected weekly with 2x108 rat erythrocytes intraperitoneally for 5 weeks. Blood samples from mice in control and REA groups were collected after 5 injections. Presence of membrane-bound autoantibodies in erythrocytes isolated from REA mice was confirmed by fluorescence microscopy after staining the erythrocytes with F(ab’)2 anti-mouse IgG-PE. Panel A shows the erythrocyte images from control and panel B the same from REA mice. The upper panels display fluorescence images and lower panels the DIC overlay (magnification 100X).