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Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse

Fig 1

Induction of autoimmune hemolytic anemia (AIHA) in mice.

Mice were injected weekly 2x108 rat erythrocytes intraperitoneally. Blood samples from mice in control and rat-erythrocyte-administered (REA) mice were collected at different time points and analyzed on an automated cell counter. Relative changes in the erythrocyte count and hemoglobin content in the REA mice over a period of 6 weeks are given in panels A and B respectively (p = 0.001 for erythrocytes, and p = 0.013 for hemoglobin after 5–6 injections, ANOVA). Presence of anti-mouse erythrocyte autoantibody was estimated by staining the erythrocytes with anti-mouse IgG/IgM-FITC following flow cytometry. Relative binding of autoantibodies (MFI) to erythrocytes over a period of 4 weeks, from 3rd-6th week, (p<0.001, ANOVA) is given in panel C. Each point on the graph represents mean ± SEM of observations. n = 10 control and 15 REA mice. *p<0.05, **p<0.01 and ****p<0.001 for comparison of the groups (Student t-test).

Fig 1

doi: https://doi.org/10.1371/journal.pone.0166878.g001