Identification of Stages of Erythroid Differentiation in Bone Marrow and Erythrocyte Subpopulations in Blood Circulation that Are Preferentially Lost in Autoimmune Hemolytic Anemia in Mouse
Mice were injected weekly 2x108 rat erythrocytes intraperitoneally. Blood samples from mice in control and rat-erythrocyte-administered (REA) mice were collected at different time points and analyzed on an automated cell counter. Relative changes in the erythrocyte count and hemoglobin content in the REA mice over a period of 6 weeks are given in panels A and B respectively (p = 0.001 for erythrocytes, and p = 0.013 for hemoglobin after 5–6 injections, ANOVA). Presence of anti-mouse erythrocyte autoantibody was estimated by staining the erythrocytes with anti-mouse IgG/IgM-FITC following flow cytometry. Relative binding of autoantibodies (MFI) to erythrocytes over a period of 4 weeks, from 3rd-6th week, (p<0.001, ANOVA) is given in panel C. Each point on the graph represents mean ± SEM of observations. n = 10 control and 15 REA mice. *p<0.05, **p<0.01 and ****p<0.001 for comparison of the groups (Student t-test).