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A Conditioned Behavioral Paradigm for Assessing Onset and Lasting Tinnitus in Rats

Fig 1

Top and side views of the behavioral testing chamber.

The horizontal waterspout is located in the front of the wire mesh chamber and connected to a syringe pump for water delivery. Speakers were mounted to the chamber walls on both sides of the waterspout, so that sound could be presented bilaterally to the animal. Sounds levels and frequencies were calibrated using a microphone (ACO Pacific, Belmont, CA) and the chamber was tested to ensure that sound presentation did not vibrate the chamber. The stainless steel grid floor was electrified to deliver footshocks. Behavioral sessions were monitored with a USB camera placed above the testing chamber.

Fig 1