A Rationally Designed TNF-α Epitope-Scaffold Immunogen Induces Sustained Antibody Response and Alleviates Collagen-Induced Arthritis in Mice
(A) Mice were immunized with chicken type II collagen (CII) emulsified with CFA on day1, and immunized with alum formulated DTT or DTNF7 on day 14, 28, and 42. (B) Arthritis incidence. (C) The average clinical scores of mice immunized with DTT or DTNF7. Data are presented as the mean clinical score ± SEM (DTT, n = 10; DTNF7, n = 9). (D) The titers of anti-mTNF-α in mice sera on day 56 measured by ELISA. Sera samples were diluted 1:200. Data are presented as the mean ± SD. (E) Examples of front and hind paws on day 28 and 56. (F) Mice body weights recorded during the entire experiments.