A Rationally Designed TNF-α Epitope-Scaffold Immunogen Induces Sustained Antibody Response and Alleviates Collagen-Induced Arthritis in Mice
(A) Thermal stability of DTT and DTNF proteins measured by DSC. Left panel, temperature dependent heat capacity of DTT and DTNF proteins. Right panel: melting temperatures of DTT and DTNF proteins. (B) SDS-PAGE analysis of the soluble fraction of DTT and DTNF proteins stored at 4°C for 2 days (Top) and 10 days (Bottom).