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Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

Fig 3

Ki-67 and F-actin staining of Huh-7.5 cells in the microwells.

Huh-7.5 cells were seeded at 0.05 × 106 cell/ml density, and after 1, 4, 7 or 10 days of incubation, cells were stained with DAPI for nucleus (blue), Alexa Fluor 488 for Ki-67 (green) and Alexa Fluor 555 for F-actin (red). DIC stands for differential interference contrast images. The bright green and red circles observed at days 4, 7 and 10 are cells that strongly express Ki-67 and F-actin (see white arrows). Scale bar is 100 μm.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0161915.g003