A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians
Egg coat matrix of R. arboreus was fixed in methanol and immunostained with the anti-P3 antibody or the anti-SMIS antibody. (a-d) Fluorescence staining with the anti-P3 antibody (a-c) and the anti-P3 antibody preabsorbed by the synthetic peptide including P3 site as a control (d). (e-h) Double-staining with the anti-P3 antibody (f) and the anti-SMIS antibody (g). (h) Merged image. Bar: 200 μm (d) and 50 μm (h).