Browse Subject Areas

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

Fig 5

Immunolocalization of SMIS in the oviduct-secreted matrix of R. arboreus.

Egg coat matrix of R. arboreus was fixed in methanol and immunostained with the anti-P3 antibody or the anti-SMIS antibody. (a-d) Fluorescence staining with the anti-P3 antibody (a-c) and the anti-P3 antibody preabsorbed by the synthetic peptide including P3 site as a control (d). (e-h) Double-staining with the anti-P3 antibody (f) and the anti-SMIS antibody (g). (h) Merged image. Bar: 200 μm (d) and 50 μm (h).

Fig 5