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A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians

Fig 4

P3 peptide binding to sperm.

Sperm of C. pyrrhogaster (a, b), R. arboreus (c, d), B. japonicus (e, f), and X. laevis (g, h) were treated with FITC-P3 (1 mM in a, c, g and 10mM in e) or FITC-P1 (1 mM in b, d, h and 10 mM in f). Bar: 10 μm.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0160445.g004