Context Matters: Distinct Disease Outcomes as a Result of Crebbp Hemizygosity in Different Mouse Bone Marrow Compartments
Fig 6
Mature Crebbp+/- myeloid cells produce excess MMP9 and have altered cytokine and gene expression profiles.
(A) Quantitative RT-PCR analysis for genes found differentially expressed in tumor-promoting myeloid cells or in MDS patients. Presented is the ratio of gene expression in Crebbp+/- myeloid populations (n = 7) compared to WT controls (n = 6), purified from the BM of 1 year-old animals. Symbols indicate statistical significance; * p<0.05; † p<0.02; ‡ p<0.01. (B) MMP9 Western Blot analysis (left panel) performed on Gr1loMac1+ cells and corresponding quantification (right panel, average + SD). (C) Heat map of transcripts of the NanoString Immunology Panel differentially expressed (adjusted P-value < 0.05) in Crebbp+/- vs WT Gr1+Mac1+ cells. The table below the heat map represents the gene ontology terms overrepresented versus the platform background and the associated genes. Genes in grey are downregulated; those in red, upregulated.