Mutations Designed by Ensemble Defect to Misfold Conserved RNA Structures of Influenza A Segments 7 and 8 Affect Splicing and Attenuate Viral Replication in Cell Culture
Total RNAs were collected 3 h post-infection (MOI = 5). The mRNA relative abundance was quantified by qRT-PCR, and normalized to the WT virus. 7MB_ED and 7PK_ED increased the amount of M1 mRNA in (A) MDCK cells and (B) A549 cells. (C) 8PK_ED and 8PK_BP decreased the amount of NEP mRNA in MDCK cells, while 8PK_BP_CP restored the splicing level.