Mutations Designed by Ensemble Defect to Misfold Conserved RNA Structures of Influenza A Segments 7 and 8 Affect Splicing and Attenuate Viral Replication in Cell Culture
(A) Pseudoknot/ hairpin equilibrium at the 3′ splice site in segment 8 . The lower helix in the pseudoknot conformation may be extended by 4 additional base pairs and a single mismatch forming between nts 494–498 and 529–533 . (B) The predicted folding of 8PK_ED. (C) Black arrow corresponds to mutation in 8PK_BP and blue arrow corresponds to compensating mutation in 8PK_BP_CP. Details are same as in Fig 1 caption.