Oxymatrine Inhibits Renal Tubular EMT Induced by High Glucose via Upregulation of SnoN and Inhibition of TGF-β1/Smad Signaling Pathway
Fig 2
Effect of OM on E-cadherin and α-SMA expression by IF-LSCM.
A, Triple-labeled LSCM images for E-cadherin (green), α-SMA (red) and nucleus (blue) of NRK52Es. The merged fluorescent images of E-cadherin, α-SMA and nucleus showed that E-cadherin (green) was distributed throughout the cytoplasm and α-SMA (red) was most prominent at the cytoplasmic membrane. The images of NG, HG and HM are at the same magnification. B, Relative Fluorescence Intensity (%) analysis demonstrated that the expression of E-cadherin was significantly decreased in the HG group as compared to the NG group (*P<0.05), but as compared to the HG group, the expression of E-cadherin was significantly increased in the HM group (#P<0.05). C, Relative Fluorescence Intensity (%) analysis demonstrated that the α-SMA expression was markedly increased in the HG group as compared to the NG group (*P<0.05), but as compared to the HG group, the expression of α-SMA was markedly decreased in the HM group (#P<0.05). Notes: NG, normal glucose control group; HG, high glucose group; HM, high glucose + OM group.