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microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration

Fig 5

Reactive oxygen species (ROS)-, IL-1β- or TNFα-stressed MG cells—involvement of NF-kB and miRNA-34a and the effects of NF-kB inhibitors or anti-miRNAs (AMs)–(A) representative Western blot of TREM2 protein levels in variably stressed MG cells both in the presence and absence of NF-kB and AMs; miRNA-34a levels were determined using microfluidic miRNA array analysis in the same sample (Fig 1); (B) note ROS-, IL-1β- or TNFα-induced increases in miRNA-34a and TREM2 protein decreases in the same sample; when present the antioxidants and/or NF-kB inhibitors PBN, CAY10512, CAPE or curcumin quenched this induction; see text for further details; similarly anti-miRNA-34a (AM-34a) but not 4 other AM species: AM-183 (or AM-9, AM-125b or AM-146a; data not shown) selectively lowered miRNA-34a levels while increasing TREM2 to 0.92 of control levels; N = 6; *p<0.05, **p<0.01 (ANOVA); NS = not significant.

Fig 5