Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging
A comparison of in vivo estimates of quenched donor fraction (A1) in the bladder and tumor of a mouse. Estimates were calculated using either all (A), the optimal ten (B) time gates and 10 evenly-spaced (C) time gates overlaid on a bright field image of the mouse. Estimates of A1 are higher in the tumor in all cases and largely similar between the three sets of time gates. Data from this figure can be found in.csv format in Supporting Information S4 File (A), S5 File (B) and S6 File (C).