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Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging

Fig 2

A comparison of parameter estimates using synthetic data across various experimental conditions.

The average relative percent error in estimation of A1 across different parameter values using all (A), the 5 optimum (B) and the 10 optimum (C) time gates shown in Table 1 is shown. Average error across all parameter values increases from 5% (all) to 6% (10) to 12% (5) with the reduction in time gates. Data from this figure can be found in.csv format in Supporting Information S1 File (A), S2 File (B) and S3 File (C).

Fig 2