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Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Fig 6

Wobble correction applied to an exemplar FtsZ ring in Caulobacter crescentus.

(a) A field of view containing several bacteria (b) Zoom of the boxed region in a, containing raw localizations of the FtsZ ring, having a tilt of ~ 9.0° (c) The same FtsZ ring as in b after application of the wobble correction with a < 1° tilt. Dotted lines in b and c guide the eye to visualize the tilt of the FtsZ ring. Scale bars: 100 nm. All data, including wobble calibration, was collected on a custom-built microscope equipped with a Nikon NA 1.49 oil immersion objective lens.

Fig 6