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Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Fig 5

Aberrations that lack rotational symmetry produce the wobble effect.

(Top row) Raw data shown at three different axial depths and corresponding wobble curves. (Middle) Phase-retrieved PSF (PR-PSF) at three axial depths and corresponding wobble curves, which are in good agreement with the raw data. (Bottom) PR-PSF with only rotationally symmetric Zernike modes. Resulting wobble calibration shows an absence of the wobble distortion.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0142949.g005