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Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Fig 3

Wobble effect on two separate systems.

(a) Wobble demonstrated on a commercial biplane setup equipped with an NA 1.42, 60X water immersion objective (UIS2 Plan Apochromat Olympus). (b) Wobble demonstrated on a system, equipped with a cylindrical lens for astigmatic, 3D imaging and a Zeiss 100X objective (Plan-Apo NA 1.46). Measurement from 4–5 beads. Error bars are standard deviation (S.D) values of the shift value.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0142949.g003