Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging
(a) Wobble demonstrated on a commercial biplane setup equipped with an NA 1.42, 60X water immersion objective (UIS2 Plan Apochromat Olympus). (b) Wobble demonstrated on a system, equipped with a cylindrical lens for astigmatic, 3D imaging and a Zeiss 100X objective (Plan-Apo NA 1.46). Measurement from 4–5 beads. Error bars are standard deviation (S.D) values of the shift value.