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A Tool for Multiple Targeted Genome Deletions that Is Precise, Scar-Free, and Suitable for Automation

Table 1

Primer sequences used to delete four candidate genes.

The PCR2_F primer is chimeric and is composed of three different parts. The bold font sequence is the region that overlaps with the PCR1 product, the italicised region represents the 40 nucleotide recombination sequence (R region) and the underlined sequence is complementary to the start of the marker gene. The PCR2_R primer consists of two parts, a region that is homologous to the target region and a region that is complementary to the marker gene (underlined).

Table 1

doi: https://doi.org/10.1371/journal.pone.0142494.t001