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Functional Analyses of Bitter Taste Receptors in Domestic Cats (Felis catus)

Fig 2

Quantitative analysis of responses of feline bitter receptors to bitter compounds.

HEK293 cells were transiently transfected with a feline bitter receptor (Tas2r2, Tas2r4, Tas2r7, Tas2r12, Tas2r38, Tas2r46, or Tas2r67), with Gα16-gust44, and assayed for their responses to bitter compounds. Data are expressed as mean ± SE percent change in fluorescence (ΔF, peak fluorescence–baseline fluorescence) compared with baseline fluorescence (F) from three independent wells. Two-tailed Student’s t-tests were performed to determine when responses from Tas2r-transfected cells were significantly different from that of mock-transfect cells. Only bitter compounds that elicited significant responses above mock-transfected cell baseline are shown in the Figure.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0139670.g002