Functional Impact of 14 Single Nucleotide Polymorphisms Causing Missense Mutations of Human α7 Nicotinic Receptor
Fig 9
Coexpression of wild type and nonfunctional mutant.
The same amount of cRNAs encoding the wild type or wild type plus mutant (in 1:1 ratio) were injected into Xenopus oocytes, and recorded with two electrode voltage clamp for ACh-induced current after 3 days of injection. A. Peak current induced by 3.16mM ACh for all groups on the 3 post-injection day. "****": P<0.0001. Each group had at least 16 oocytes. B. Averaged concentration response relationships of the wild type and wild type plus mutant as indicated. Each group is the average of six oocyte data. C. EC50 values were derived by fitting individual concentration-response curves in B. pEC50 values (negative logEC50) are used to plot the data. ("**", “***”, “****”: p<0.01, P<0.001, or P<0.0001). D. Linear regression analysis for the relationship between Hill slope and LogEC50.