Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes
Muscle biopsies from two representative animals, one homozygous (homoz.) for a frameshift mutation and another heterozygous (heteroz.) for a frameshift mutation and a WT copy in the second allele (# 43 and 44, respectively, the numbers of animals correspond to those of Fig 3) analyzed by western blot using an anti-MSTN monoclonal antibody. After stripping of the anti-MSTN antibody from the membrane, an anti-GAPDH was used as loading control. One representative western blot experiment out of three performed with the same results.