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Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

Fig 2

Genotype of lambs born following microinjection of sgRNA for MSTN exon 1 and Cas9.

DNA was extracted from lambs’ skin biopsies one week after birth, with the exception of lambs #43 and #49 that were stillborn and were biopsied 1–2 hours after delivery. A) Results from PCR amplifying 634 bp. Additional larger bands represent formation of heteroduplex DNA double strands with mismatches between the two strands resulting in slower migration during electrophoresis. B) T7EI assay of lambs produced by zygote injection of CRISPR/Cas9 mRNA, indicating numbers of mutant animals with bands of 255, 379 and 634 bp (#40, 43, 44, 47, 49, 50, 51, 56, 57 and 60) and WT animals with only one band of 634 bp (#41, 42, 45, 46, 48, 52, 53, 54, 55, 58, 59 and 61).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0136690.g002