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PEAK1 Acts as a Molecular Switch to Regulate Context-Dependent TGFβ Responses in Breast Cancer

Fig 6

PEAK1 potentiates TGFβ-induced Src/MAPK signaling in a fibronectin-dependent manner.

(A and C) MCF7-Vector and-PEAK1 cells were plated on the indicated substrates (i.e., Fibronectin—F, Collagen—C, Laminin—L, or Plastic–P). Cells were then serum starved for 8 hours, then stimulated with TGFβ for 30 minutes. Lysates were collected and Western blot analysis was done for indicated proteins and phospho-proteins. (B and D) Band intensity ratio for phospho-protein to total protein was calculated for western blots from A and C, respectively.

Fig 6