PEAK1 Acts as a Molecular Switch to Regulate Context-Dependent TGFβ Responses in Breast Cancer
(A-D) MCF7-Vector and–PEAK1 or CA1h-shCntrl and–shPEAK1 cells were chronically treated with TGFβ while being cultured on plastic. (A and C) Single cell migration assays were performed on these cells after plating on the indicated substrates with 3 images per condition being collected every 10 minutes for 24 hours. Cells were tracked using Fiji software to determine Displacement (μm) (left y-axis) and Velocity (μm/min) (right y-axis). Two-way ANOVA statistical analysis was performed using Prism. (B and D) 10 representative cell tracks for each of the indicated cell populations are shown when cells were migrating on fibronectin. *** indicate p-values < 0.001.