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PEAK1 Acts as a Molecular Switch to Regulate Context-Dependent TGFβ Responses in Breast Cancer

Fig 2

PEAK1 potentiates fibronectin/TGFβ-induced EMT-like responses.

(A-C) MCF7-Vector and-PEAK1 cells were plated on 3ug/mL Fibronectin (F), Collagen (C) or Laminin (L), or plated on Plastic (P) and treated chronically with TGFβ. (A) Western blot analysis for E-cadherin, N-cadherin, and α-tubulin was performed after two weeks in culture. (B) Western blot band quantification from (A) using densitometry analysis. (C) Micrographs of cells after two weeks in culture. Yellow arrowhead points to a typical cell that is not packed tightly into epithelial colonies and is mesenchymal. (D & E) qPCR for E-Cadherin and Vimentin expression (D) and micrographs (E) from CA1h-shCntrl and-shPEAK1 cells plated on fibronectin and treated with TGFβ or vehicle control for 48 hours. (Scale bar: 90μm). Yellow arrowhead points to a typical cell that is not packed tightly into epithelial colonies and is mesenchymal.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0135748.g002