Convection-Enhanced Delivery of Carboplatin PLGA Nanoparticles for the Treatment of Glioblastoma
Carboplatin NP are less toxic to neurones compared to the free drug. Primary rat brain hippocampal cultures were dosed with either carboplatin (0.03mg/ml) or carboplatin NP (1mg/ml) and assayed after 72 hours of culture. (A) MTT analysis shows significant increase in cell viability with carboplatin NP (p<0.001). (B) Carboplatin alone causes deregulation of the neurones and loss of the glial cells, whilst carboplatin NP retained neuronal connections and glial cells. Immunofluorescent analysis of the neurones (B3tubulin; green) and glial cells (GFAP; red). Cells were counterstained with DAPI to visualise cell nuclei (blue) (Scale bar; 100μm).