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Convection-Enhanced Delivery of Carboplatin PLGA Nanoparticles for the Treatment of Glioblastoma

Fig 3

In vitro neurotoxicity in primary rat hippocampal cultures.

Carboplatin NP are less toxic to neurones compared to the free drug. Primary rat brain hippocampal cultures were dosed with either carboplatin (0.03mg/ml) or carboplatin NP (1mg/ml) and assayed after 72 hours of culture. (A) MTT analysis shows significant increase in cell viability with carboplatin NP (p<0.001). (B) Carboplatin alone causes deregulation of the neurones and loss of the glial cells, whilst carboplatin NP retained neuronal connections and glial cells. Immunofluorescent analysis of the neurones (B3tubulin; green) and glial cells (GFAP; red). Cells were counterstained with DAPI to visualise cell nuclei (blue) (Scale bar; 100μm).

Fig 3

doi: https://doi.org/10.1371/journal.pone.0132266.g003