Convection-Enhanced Delivery of Carboplatin PLGA Nanoparticles for the Treatment of Glioblastoma
(A) Carboplatin NP showed increased cytotoxicity over 48 hours. The cytotoxic effect of the carboplatin nanoparticles (Carboplatin NP) were assessed by MTT assay in UPAB and SNB19 human glioblastoma multiforme (GBM) cell lines. For UPAB, 0.18mg/ml and for SNB19, 0.03mg/ml carboplatin was used as these concentrations represent the IC50 after 72 hours. Paired t-test statistical analysis comparing cytotoxicity revealed significant differences for SNB19 at 24hours (p = 0.001) and 48 hours (p = 0.004), indicated by asterix. (B) Uptake of fluorescein-labelled carboplatin NP (green) occurs within 24 hours of dosing. Cells were dosed and fixed after defined periods of culture. Cells were stained with phalloidin (red), to visualise actin cytoskeleton and DAPI (blue) for cell nuclei. Uptake into cells is indicated by white arrows.