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A Genetic Porcine Model of Cancer

Fig 2

Inducible expression of KRASG12D and TP53R167H is transforming and tumorigenic.

(a) RT-PCR analysis of KRASG12D and TP53R167H mRNA expression in the fibroblast cell lines from each of the 4 transgenic clones treated with AdCre or AdGFP. (b) Comparison of cell morphology between AdCre and untreated control cells in culture, stained with H&E. (c) Normalized MFU measured by FACS at time points following Carboxyfluorescein succinimidyl ester (CFSE) dye loading of cells. (d) Graphical analysis of the mean number of migrating cells from triplicate plating of each of the 4 cell lines. (e) Graphical analysis of the mean number of colonies growing in soft agar for each cell line from triplicate plating. (c-e: all data points are the mean of the 4 cell lines derived from pigs 63–1, 63–2, 63–3, 63–4; error bars = SD; *p-value ≤ 0.05; **p-value ≤ 0.01).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0128864.g002