Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Prevalence and Characterization of Murine Leukemia Virus Contamination in Human Cell Lines

Fig 2

Direct DNA Sanger sequencing chromatograms showing sequence polymorphisms in the human cell lines COLO-677, KYSE-70, HN, and the mouse- derived cell line 32D.

COLO-677 and KYSE-70 contain single sequences of MLV contaminants, whereas HN and 32D show identical chromatograms, indicating that the DNA of the human cell line was contaminated with mouse DNA. A single nucleotide deletion (arrows) results in a sequence shift leading to an overlap of two MLV sequences present in the mouse genomic DNA.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0125622.g002