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Prevalence and Characterization of Murine Leukemia Virus Contamination in Human Cell Lines

Fig 2

Direct DNA Sanger sequencing chromatograms showing sequence polymorphisms in the human cell lines COLO-677, KYSE-70, HN, and the mouse- derived cell line 32D.

COLO-677 and KYSE-70 contain single sequences of MLV contaminants, whereas HN and 32D show identical chromatograms, indicating that the DNA of the human cell line was contaminated with mouse DNA. A single nucleotide deletion (arrows) results in a sequence shift leading to an overlap of two MLV sequences present in the mouse genomic DNA.

Fig 2