High Affinity Antibodies against Influenza Characterize the Plasmablast Response in SLE Patients After Vaccination
Antibodies were tested for polyreactivity against dsDNA, LPS and insulin by ELISA and for HEp-2 reactivity by ELISA and IFA. (A) Pie charts represent the frequency of polyreactive and non-polyreactive influenza-positive antibodies for each individual. Numbers in the center indicate number of influenza-positive antibodies for each individual. Dot plot (right) summarizes the percent of polyreactive antibodies from the pie charts; each symbol represents an individual. (B) Similar to (A), except for HEp-2 ELISA reactivity. (C) Distribution of the HEp-2 IFA scores for each individual is shown and the dot plot summarizes the percent of antibodies with a score of 2 and above for each individual. (D) Distribution of antibodies with HEp-2- only, poly- only and both reactivities is shown for pooled influenza-positive antibodies in the pie charts. Values in pie chart segments are percentages. Statistical significance of the difference between the two cohorts was determined by the Chi-squared test (*p<0.05; **p<0.001; ***p<0.0001). Dot plots show the percent of each type of reactivity for each individual. (E) Average HEp-2 IFA score of all influenza-positive antibodies for each individual is plotted. Medians were compared by Mann-Whitney test in A-C, D (dot plot), E; and data are representative of at least three independent replicates.