Novel Pactamycin Analogs Induce p53 Dependent Cell-Cycle Arrest at S-Phase in Human Head and Neck Squamous Cell Carcinoma (HNSCC) Cells
(A-D):48-h treatment with TM-025/TM-026 showed significant (P<0.0001; depicted with *) dose-dependent decrease in the levels of Ki67, a cell proliferation marker, in SCC25 (A and B) and SCC104 (C and D) cells. All experiments were performed in five replicates, and the results are presented as means (±SEM). (E-F): 48 h treatment with either of the PCT analogs exhibited a dose-dependent (0, 1, 10 and 50 nM) decrease in the percentage of actively proliferating (BrdU+) cells in SCC25 (E) and SCC104 (F) cells. Results were significant at P<0.0001 (n = 5), and depicted with *.