Effect of Lysyl Oxidase Inhibition on Angiotensin II-Induced Arterial Hypertension, Remodeling, and Stiffness
Fig 5
Effect of Ang II and BAPN on LOX, collagen, and cross-linking in aortas.
The effect of LOX is not confined to the crosslinking of collagen and elastin and therefore we examined the effect of LOX on gene expression of other key ECM proteins and enzymes. A, Lysyl-oxidase enzymatic activity measured from the lower thoracic aorta represented by the production of H2O2 and detected by Amplex red oxidation. B, The total collagen content using hydroxyproline concentrations assuming collagen contains 13.5% hydroxyproline. C, Cross-linked collagen, using cyanogen bromide as digestion. D, Percent of collagen cross-linking. n = 6. *P<0.05 compared to control, ƗP<0.05 compared with BAPN group, #P<0.05 compared with Ang II group at their respective day.