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Analysis of Antibody Aggregate Content at Extremely High Concentrations Using Sedimentation Velocity with a Novel Interference Optics

Fig 8

Simulation of the impact of the third term of the Svensson equation (7) for a typical dataset from this study for different values of r.

For easier interpretation, we have transformed ΔS into fringes, using our laser wavelength of 660 nm. The plot shows that in the center of the boundary, at a refractive index gradient of 800 fringes/cm, a contribution of 1 fringe to a total signal of approximately 80 fringes is attained only for a vast error in r. The inset shows a comparison for 1-cm-cells at the same gradient with a much larger impact and clearly visible Wiener skewing of the total signal.

Fig 8

doi: https://doi.org/10.1371/journal.pone.0120820.g008