Actin Cytoskeleton Regulation of Epithelial Mesenchymal Transition in Metastatic Cancer Cells
Fig 4
Cyt D treatment induces nuclear-cytoplasmic distribution of SNAI 1 and SMAD1/2/3.
(A) Du145, MDA-MB-231, MDA-MB-435, HT-1080, U251 and U87 cells were untreated (Control) or treated with 100 μM Cyt D (Cyt D treated) for 12 h and immunofluorescently labelled for Smad1/2/3 and SNAI 1 as well as Hoechst 33342 for nuclear staining. Representative images for MDA-MB-231 cells are shown. (B) Cells were scored for nuclear distribution of Smad1/2/3 and SNAI 1 in control and Cyt D treated cells. Results are presented as percentage of total cell showing nuclear distribution for these two proteins. Scale: 10 μm; **, p<0.01, * p<0.05; relative to untreated control for each cell line.