Single Particle Fluorescence Burst Analysis of Epsin Induced Membrane Fission
(a) BAS histograms of 200 nm-diameter, TopFluor-labeled, (5%) PtdInsP(4,5)P2 Folch liposomes before (red) and after incubation at 37°C for 40 min with 1 μM (green), 5 μM (purple), and 10 μM (blue) full-length epsin. (b) Heat-map representation of the fractional intensity for each reaction shown in (a). (c) Comparison of ENTH and epsin activity. BAS histograms of starting liposomes (red), and liposomes incubated at 37°C for 90 min with 2 μM ENTH (blue) or full-length epsin (green). The data shown is representative of three experimental replicates.