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Single Particle Fluorescence Burst Analysis of Epsin Induced Membrane Fission

Fig 4

Dose dependence of ENTH-mediated vesiculation.

(a) BAS histograms of 200 nm-diameter, TopFluor-labeled, (5%) PtdInsP(4,5)P2 Folch liposomes before (red) and after incubation at 37°C for 20 min with 500 nM (blue), 1 μM (green), 5μM (purple), and 10 μM (cyan) ENTH. (b) Heat-map representation of the fractional intensity for each reaction shown in (a). The data shown is representative of three experimental replicates.

Fig 4