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Single Particle Fluorescence Burst Analysis of Epsin Induced Membrane Fission

Fig 2

BAS analysis of liposomes vesiculated by the ENTH domain of epsin.

Fluorescent burst data for 400 nm-diameter, TopFluor-labeled, (5%) PtdInsP(4,5)P2 Folch liposomes incubated at 37°C for 40 min before (a) and after addition of 2 μM ENTH (b). Fluorescent burst data for 200 nm-diameter liposomes incubated at 37°C for 40 min before (c) and after addition of 2 μM ENTH (d). (e) BAS histograms generated from starting 200 nm liposomes before (red) and after addition of ENTH (blue; insets indicate resolution of small particles in a 10-fold dilution of the same reaction). (f) FCS profiles of liposomes extruded to 100 nm (green), 50 nm (cyan) and the end products (purple) of the fission reaction of 200 nm liposomes from (d). The data shown is representative of three experimental replicates.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0119563.g002